GB19170-2003
Pure culture of Lentinula edodes
香菇菌种
GB 19170-2003 is a current mandatory Chinese national standard for Pure culture of Lentinula edodes. It appears in the Food compliance cluster with a product safety focus and has an implementation date of Dec 1, 2003.
Applicability depends on product scope, certification route, competent authority notices and transition rules. Requst for the translation or verify source before using this record in a compliance decision.
Compliance overview
Scope and applicability
This standard is mandatory for the production, distribution, and use of Shiitake mushroom (Lentinula edodes) pure cultures in China. It specifically applies to the three-tier propagation system: stock cultures (mother spawn), pre-culture spawn, and cultivation spawn. It is essential for any enterprise involved in the commercial breeding or trading of Shiitake mushroom spawns. The standard does not cover other edible mushroom species, which are regulated under separate standards like GB 19172-2003 for oyster mushrooms.
Technical requirements
The standard specifies comprehensive quality requirements across three levels of Shiitake mushroom spawns. For stock cultures, it mandates specific sensory characteristics such as mycelium density, color uniformity, and the absence of sector mutations or high-temperature inhibition lines. Microbiological purity is strictly enforced, requiring the presence of clamp connections and the complete absence of contaminants. For pre-culture spawn and cultivation spawn, the standard outlines container specifications, mycelium growth vigor, and sensory parameters. It explicitly prohibits the presence of antagonism phenomena, foreign fungal colonies, and premature primordia (fruiting body initials) formation. The standard also defines acceptable mycelial growth speeds under specific temperature conditions to ensure viability. Furthermore, the standard requires that stock cultures undergo rigorous cultivation trials to verify their agronomic traits and commercial viability before being approved for multiplication or sale. Suppliers must prove the biological efficiency and morphological stability of the strains. Packaging, labeling, and storage conditions, including specific temperature ranges and shelf-life limits, are also strictly regulated to maintain spawn vitality during transportation.
Testing methods
Testing involves a combination of sensory, microbiological, and agronomic evaluations. Sensory inspections are primarily conducted via visual examination and olfactory checks to assess container integrity, mycelium appearance, and odor. Microbiological testing utilizes optical microscopy (minimum 10x40 magnification) to verify mycelial morphology and clamp connections, alongside culture-based methods to detect bacterial and fungal contamination using nutrient broth and PDA media. Growth speed tests measure the time required for mycelium to colonize the substrate under controlled temperatures. Additionally, cultivation trials are conducted to evaluate biological efficiency, yield, and morphological characteristics against a reference strain.
FAQs
What products are regulated under GB 19170-2003?
GB 19170-2003 regulates Shiitake mushroom (Lentinula edodes) pure cultures, specifically covering the three-tier propagation system: stock cultures (mother spawn), pre-culture spawn, and cultivation spawn used for commercial production.
What are the key microbiological requirements for Shiitake spawn under this standard?
The standard requires that the mycelium be robust, full, and uniform, with the presence of clamp connections. It strictly mandates the complete absence of any bacterial or fungal contaminants.
How is the agronomic quality of the mother spawn verified?
Mother spawn must undergo cultivation trials where it is used to create pre-culture spawn and then cultivated into mushroom sticks. The resulting biological efficiency, yield, and mushroom morphology are compared against the original strain; any significant delay in growth or deviation in form results in disqualification.
The summary above provides a high-level overview of GB 19170-2003. To ensure complete compliance for your Shiitake mushroom spawn entering the Chinese market, a precise and professional translation of the full technical specifications is essential. Purchase our high-quality English translation of GB 19170-2003 today to access all detailed parameters, tables, and testing procedures.
Applicability hints
- Type
- Product
- CCS
- B39
- ICS
- 65.020.20
- Competent department
- Ministry of Agriculture and Rural Affairs
- Technical committee
- Ministry of Agriculture and Rural Affairs
Relationships
Replaced by:GB 19170-2026 (Upcoming)