GB19171-2003
Pure culture of Agaricus bisporus
双孢蘑菇菌种
GB 19171-2003 is a current mandatory Chinese national standard for Pure culture of Agaricus bisporus. It appears in the Food compliance cluster with a product safety focus and has an implementation date of Dec 1, 2003.
Applicability depends on product scope, certification route, competent authority notices and transition rules. Requst for the translation or verify source before using this record in a compliance decision.
Compliance overview
Scope and applicability
This standard applies to the production, distribution, and use of pure cultures of Agaricus bisporus (button mushroom spawn) in China. It covers the three-tier propagation system: stock culture (mother spawn), pre-culture spawn (original spawn), and cultivation spawn. It is mandatory for all entities involved in the commercial mushroom spawn supply chain. The standard references NY/T 528-2002 for container specifications and general production technical rules.
Technical requirements
The standard specifies comprehensive quality requirements for three levels of Agaricus bisporus spawn. For stock cultures, it mandates specific sensory characteristics, microbial purity, and mycelial growth rates on PDA media. Furthermore, suppliers must verify genetic and agronomic traits through esterase isoenzyme typing and fruiting trials before distribution. For pre-culture spawn and cultivation spawn, the standard dictates container integrity, sensory parameters like mycelium density and absence of antagonism, and strict microbial limits to prevent contamination. The standard also sets specific limits for mycelial growth speed, requiring cultures to fill containers within a defined timeframe under optimal temperatures. GB/T 12728-1991 terminology is utilized for biological efficiency metrics.
Testing methods
Testing procedures include sensory inspection via visual observation and olfactory checks. Microbiological examinations utilize optical microscopy for mycelium and contaminant identification, alongside specific bacterial and fungal cultivation tests using GB/T 4789.28 protocols. Genetic verification involves slab polyacrylamide gel electrophoresis for esterase isoenzyme typing. Agronomic properties are validated through controlled cultivation trials to assess fruiting capability and biological efficiency.
FAQs
What products are regulated under GB 19171-2003?
GB 19171-2003 regulates pure cultures of Agaricus bisporus, commonly known as button mushroom spawn, including stock cultures, pre-culture spawn, and cultivation spawn.
What are the key genetic testing requirements for stock cultures?
Stock cultures must undergo esterase isoenzyme typing using polyacrylamide gel electrophoresis to confirm genetic identity. They must also pass fruiting trials to verify agronomic traits and biological efficiency before sale.
Are there specific storage and transportation requirements?
Yes, the standard specifies temperature-controlled storage (e.g., 5°C±1°C for stock cultures) and requires refrigerated transport (2°C to 20°C) when ambient temperatures exceed 30°C to maintain spawn viability.
The summary above provides a high-level overview of GB 19171-2003. To ensure complete compliance for your mushroom spawn products entering the Chinese market, a precise and professional translation of the full technical specifications is essential. Purchase our high-quality English translation of GB 19171-2003 today to access all detailed parameters, tables, and testing procedures.
Applicability hints
- Type
- Product
- CCS
- B39
- ICS
- 65.020.20
- Competent department
- Ministry of Agriculture and Rural Affairs
- Technical committee
- Ministry of Agriculture and Rural Affairs
Relationships
Replaced by:GB 19171-2026 (Upcoming)