GB19172-2003
Pure culture of Pleurotus ostreatus
平菇菌种
GB 19172-2003 is a current mandatory Chinese national standard for Pure culture of Pleurotus ostreatus. It appears in the Food compliance cluster with a product safety focus and has an implementation date of Dec 1, 2003.
Applicability depends on product scope, certification route, competent authority notices and transition rules. Requst for the translation or verify source before using this record in a compliance decision.
Compliance overview
Scope and applicability
This standard applies to the production, distribution, and use of pure cultures of Pleurotus ostreatus (oyster mushroom) and related species within the Pleurotus genus, including Pleurotus sapidus, Pleurotus cornucopiae, Pleurotus pulmonarius, and Pleurotus florida. It is mandatory for all manufacturers and distributors of these mushroom spawns in China. The standard specifically covers the three-tier cultivation system: stock cultures (mother spawn), pre-culture spawn (original spawn), and cultivation spawn.
Technical requirements
The standard specifies comprehensive quality requirements for three levels of mushroom spawns. For stock cultures, it mandates specific container specifications, sensory requirements (such as mycelial density and purity), and microbiological criteria including the presence of clamp connections and absence of contaminants. For pre-culture spawn and cultivation spawn, the standard details sensory parameters like container integrity, mycelial growth uniformity, and the absence of antagonism or high-temperature inhibition lines. Any spawn exhibiting contamination or abnormal growth must be rejected. Additionally, the standard sets specific mycelial growth speed parameters under defined temperature conditions (25°C±2°C) for different culture media. It also requires that stock cultures undergo cultivation trials to verify agronomic and commercial traits, with a minimum biological efficiency threshold for yield validation.
Testing methods
Testing involves sensory inspection through visual observation and olfactory checks. Microbiological examinations require the use of optical microscopes (magnification ≥10x40) to observe hyphal morphology and clamp connections. Contamination tests include bacterial detection using nutrient broth and mold detection using PDA media. Furthermore, mycelial growth speed is evaluated by measuring the time required to fully colonize the substrate under controlled temperatures. Comprehensive agronomic trait testing is mandatory for stock cultures.
FAQs
What products are regulated under GB 19172-2003?
GB 19172-2003 regulates pure cultures (spawns) of Pleurotus ostreatus and related species in the Pleurotus genus, covering stock cultures, pre-culture spawn, and cultivation spawn used in mushroom production.
What are the key quality parameters for mushroom spawns under this standard?
Key parameters include sensory characteristics (container integrity, mycelial appearance), microbiological purity (absence of contaminants, presence of clamp connections), mycelial growth speed, and for stock cultures, verified agronomic traits with a minimum biological efficiency.
Are there specific storage and transportation requirements in GB 19172-2003?
Yes, the standard mandates strict storage temperatures (e.g., 5°C±1°C for stock cultures) and maximum storage periods. Transportation requires measures against shock, sunlight, and contamination, including the use of refrigerated vehicles when temperatures exceed 30°C.
The summary above provides a high-level overview of GB 19172-2003. To ensure complete compliance for your products entering the Chinese market, a precise and professional translation of the full technical specifications is essential. Purchase our high-quality English translation of GB 19172-2003 today to access all detailed parameters, tables, and testing procedures.
Applicability hints
- Type
- Product
- CCS
- B39
- ICS
- 65.020.20
- Competent department
- Ministry of Agriculture and Rural Affairs
- Technical committee
- Ministry of Agriculture and Rural Affairs
Relationships
Replaced by:GB 19172-2026 (Upcoming)