GB7300.503-2023
Feed additives —Part 5：Live microorganisms — Enterococcus faecium
饲料添加剂 第5部分：微生物 屎肠球菌
GB 7300.503-2023 is a current mandatory Chinese national standard for Feed additives —Part 5：Live microorganisms — Enterococcus faecium. It appears in the Food compliance cluster with a product safety focus and has an implementation date of Jun 1, 2024.
Applicability depends on product scope, certification route, competent authority notices and transition rules. Requst for the translation or verify source before using this record in a compliance decision.
Compliance overview
Scope and applicability
This standard applies to feed additive products containing Enterococcus faecium, which are produced through liquid or solid-state fermentation and subsequent drying processes. It is mandatory for manufacturers producing microbial feed additives intended for the Chinese animal nutrition market. It does not apply to other live microorganisms or non-microbial feed additive categories.
Technical requirements
The standard outlines the physical, chemical, and biological parameters for Enterococcus faecium. It specifies that raw materials such as carriers and diluents must comply with the national feed hygiene standard GB 13078. The product must present as a uniform powder or granule without foreign matter or off-odors. For the microbial strain itself, it must meet specific morphological, physiological, biochemical, and molecular biology characteristics. The standard strictly regulates the viable bacteria count and moisture content to ensure product stability and efficacy. Furthermore, the standard sets strict hygiene limits for heavy metals (such as lead, arsenic, mercury, and cadmium) and mycotoxins (including aflatoxin B1 and zearalenone). Pathogenic microorganisms like Salmonella must not be detected. A critical safety requirement is the ampicillin sensitivity parameter. The strain must demonstrate susceptibility to ampicillin, ensuring it does not carry transferable antibiotic resistance genes.
Testing methods
The standard mandates specific testing protocols to verify compliance. Strain identification involves morphological observation on selective media, physiological and biochemical tests, and 16S rRNA gene sequence analysis. Viable bacteria counting is conducted using specialized MRS media. Physicochemical and hygiene parameters are tested using referenced national standards, such as GB/T 6435 for moisture and GB/T 13080 for lead. Microbial contamination levels for Salmonella and coliforms are evaluated using corresponding GB/T methods. Additionally, the ampicillin Minimum Inhibitory Concentration (MIC) is determined following ISO 20776-1:2006.
FAQs
What products are regulated under GB 7300.503-2023?
This standard applies specifically to feed additive products containing Enterococcus faecium, which are produced through liquid or solid-state fermentation and subsequent drying processes. It is mandatory for all manufacturers producing and selling this specific microbial feed additive in China.
Are there any specific safety or antibiotic resistance requirements in GB 7300.503-2023?
The standard introduces updated and highly specific requirements for strain identification, including 16S rRNA molecular biology sequencing, and sets a strict limit for ampicillin sensitivity (MIC ≤ 2mg/L) to ensure the safety and efficacy of the Enterococcus faecium strain.
How does GB 7300.503-2023 differ from ISO standards?
The standard references several national methodologies for testing hygiene and physicochemical parameters. Notably, for evaluating the ampicillin MIC value, it directly references ISO 20776-1:2006 for the testing procedure.
The summary above provides a high-level overview of GB 7300.503-2023. To ensure complete compliance for your feed additive products entering the Chinese market, a precise and professional translation of the full technical specifications is essential. Purchase our high-quality English translation of GB 7300.503-2023 today to access all detailed parameters, tables, and testing procedures.
Applicability hints
- Type
- Product
- CCS
- B46
- ICS
- 65.120
- Competent department
- Ministry of Agriculture and Rural Affairs
- Technical committee
- Ministry of Agriculture and Rural Affairs